top.jpg - 4914 Bytes

This is my method of pollen storage, seems to work well for me. I am sure you could change it some, the main thing is get the pollen dried and stored and kept that way until used. I am sure humidity changes would ruin it if not protected and frozen though. I know some use gel caps instead of tubes.

I use magnifying reading glasses(getting older), can see better using them. I also use a pair of tweezers with an angle on them(see photo below). I make a tag for each plate, easy to forget what you have if you are doing more than one plant. I hold the plate under the anthers and sort of snap them off with maybe 1/4-3/8" of the filament, this helps with getting them into the centrifuge tubes and also something to hang on to when using the pollen later. You can press down on the style and slide the tweezers between it and the filaments.

pollen1.jpg - 58978 Bytes

I write numbers on the tubes with a sharpie and on a paper which I later add to excel to keep track.

pollen2.jpg - 58978 Bytes

I take the tweezers and insert each anther into the micro tube and put it in silica gel. I put about 6 anthers in each tube, doesn't really matter how many. I found loose gel on ebay. I leave the caps open, insert them into the gel like shown, and shut the lid on the tupperware container over night.

pollen3.jpg - 58978 Bytes

Then in 24 hours I take the tubes out, close them, and place them in another tupperware container in the freezer for long term storage. This container has a lid as well which I close after placing the tubes in it.

pollen4.jpg - 58978 Bytes

Home




frontpage hit counter
asp hit counter